This instruction material assumes you have viewed the videos on YouTube. The manual is more extensive, covering more than the material presented on the videos. It also shows how the same steps can be done in Image J and GIMP (a free, post-processing software modeled after Photoshop).
PDF for Photoshop Methods
New material is added from time to time. Please check list to left under Photoshop Instruction Materials for new additions.
Topics covered include:
Photoshop Set Up: Setting up Photoshop, Recording Steps with History Log.
Opening Images: Opening Problem Images (indexed color & 10- 12- 14-bit images in a 16-bit format), , Opening Movies in Photoshop, Opening Series of Images to Layers or Z-Project, Montaging (Photostitching) in Photoshop, Extended Focus in Photoshop
Conversion Steps: Converting Color to Grayscale Color Brightfield, Color to Grayscale Fluorescent Images, Rotate Flip and Straighten, Crop and Cropping More than One Image
Uneven Illumination: Checking for Uneven Illumination, Uneven Illumination Flatfield Correct, Uneven Illumination Correct by Using Image Itself, Uneven Illumination Phase DIC TEM
DeNoising: Reduce Remove Outliers, Reduce Surface Blur, Reduce Image Averaging, Reduce Image Size Method, Remove DeInterlacing (for video), Reduce Color Noise (color fringing)
Color and Tonal Adjustment: Tonal Adjustment Manual Method, Tonal Adjustment Auto Method, Tonal Adjustment for Densitometry (linear histogram shift), Tonal Adjustment Equalize, Tonal Adjustment Gamma, Color Brightfield Tonal Adjustment Manual, Color and Tonal Adjustment Match Color or Tone
Visualization: Pseudocolor, Reduce Number of Tones or Colors, Colorize Fluorescence, Colocalization CoExistence, Merge Fluorescence
To Output: Scale Bars, Set Resolution, Sharpening.