consulting banner

How Jerry can Improve Imaging and Quantitation
  • Setting up and evaluating new imaging equipment in a lab
  • Establishing best-practices for your imaging and analysis
  • Streamlining your imaging workflow
  • Implementing best-practices for research integrity
  • Improving macrophotography image quality and consistency
  • Providing polarization solutions for glare free images
  • Instituting methods for post-processing, including pseudocolor
  • Authoring manuscripts, white papers, and reports
  • Solving morphometric & optical density measurement challenges
  • Bringing new products & software to the microscopy community
  • Color Brightfield and Fluorescence Imaging and Analysis expert
  • Training in macro- and micro-photography and post-processing
  • Designing software for analysis and computer aided diagnosis
  • Photographing small and microscopic objects and samples
  • Testifying in legal disputes regarding scientific misconduct
  • Calibrating and setting standards for imaging workflows
link for Courses home link image integrity link products link consulting link support
 
PHOTOGRAPHY
MICROSCOPY | MACROSCOPY
 
IMAGE INTEGRITY ANALYSIS
PUBLISHERS | LAW FIRMS | COLLEGES
 
QUANTITATION
COMPUTER AIDED ANALYSIS | MACHINE VISION
 
 

Don't believe you can't get the picture you want... you will.

Contact Jerry Sedgewick for assistance with light microscopy (color brightfield, confocal, fluorescence, polarizing, special techniques), and small object photography via a camera, scanner or stereo microscope. Jerry can take images for you, train you in methods for getting the image you want, or design a camera and lighting system specific to your needs. Contact form.

Fluorescence Imaging Expert
Microscopy and Macroscopy (Stereo Microscope) expert
Confocal, multiphoton imaging
Glare-free, shadow-free imaging
Difficult objects imaging: glass, plastic, detritus on surfaces
Imaging done with calibration standard, including color calibration
Imaging done for Densitometry purposes
Training
Design of imaging systems

 


Example Images

single grain
Single grain image: no shadows

braided wire
Braided wire: no glare

extended focus
High Magnification Image: Extended Focus

cross section tubes
Cross Section Tubes: Difficult Lighting

 

Contact Jerry Sedgewick for analysis of images when there are allegations of scientific misconduct. Mr. Sedgewick has decades of experience working with scientific images in the Life Sciences. These include fluorescence images, gels, blots, westerns, northerns, southerns, phosphor samples, chemiluminescence, and so on. Mr. Sedgewick is an expert at Photoshop, having written two books on Photoshop for scientific imaging.

Jerry Sedgewick CV pdf

We use unique software and methods to accomplish the following:

checkmark Pattern Recognition
checkmark Metadata Extraction
checkmark Comparative Feature Analysis
checkmark Source Imaging System Analysis

Can You Find the Added Band?
Here's a potential approach to finding a user-created band:

added band to gel
Gel with band added in Photoshop

metadata confirming dates
Metadata confirming use of Photoshop

color gradient approach
Color gradient yields no useful information

filtered image
Filtered image shows that bottomost band appears unlike others

equalized image
Equalized image confirms difference between bottomost image and others (note crisp edge)

Bottom band is believed to be created by the user.

Contact Jerry Sedgewick (at) gmail.com for more information.

 

 




 

When others haven't figured it out... we can

Contact Imaging and Analysis for the development of stand alone systems for machine vision, Computer Aided Diagnosis (CAD), and computer aided analysis of images. Once the system is designed and tested to your specifications, we work with a team of experts in software and hardware development. We can also assist with smaller jobs, such as the development of routines for image measurement in Photoshop and Image J, or do measurements for your FDA studies, etc. Contact Form.

Image Analysis for Biological, Medical and Materials.

Why We Can Solve Problems Others Can't
We are rare in the industry because we understand the 3 components to image analysis: Image Acquisition and Lighting; Post-Processing; and Quantitation. Few understand all three.

Jerry Sedgewick has worked on the ground floor with thousands of images: it's not just from book learning.

We understand that most images suffer from 3 problems: noise, uneven illumination and varied exposures. We have solutions.

tortuosity
Method developed for determining tortuosity in retinal vessels by using overlays (above). Original image in color from fundus camera

vessel length
Method for measuring vessel length from autofluorescent vessels using skeletonization

picks per inch

Automated method that exceeded accuracy of manual count by 7 times

 

 

Selected Past and Present Clients

past and current clients